토끼 두개골 결손부에서 전기 방사된 나노실크-수산화인회석 복합체를 이용한 골재생 효과에 대한 연구
Electrospun Silk Nano-Fiber Combined with Nano-Hydoxyapatite Graft for the Rabbit Calvarial Model
계준영, 김성곤, 김민근, 권광준, 박영욱, 김좌영, 이민정, 박영환,
소속 상세정보
계준영 ( Kye Jun-Young ) - 강릉원주대학교 치과대학 구강악안면외과학교실
김성곤 ( Kim Seong-Gon ) - 강릉원주대학교 치과대학 구강악안면외과학교실
김민근 ( Kim Min-Geun ) - 강릉원주대학교 치과대학 구강악안면외과학교실
권광준 ( Kwon Kwang-Jun ) - 강릉원주대학교 치과대학 구강악안면외과학교실
박영욱 ( Park Young-Wook ) - 강릉원주대학교 치과대학 구강악안면외과학교실
김좌영 ( Kim Jwa-Young ) - 한림대학교 성심병원 구강악안면외과학교실
이민정 ( Lee Min-Jung ) - 서울대학교 바이오시스템·소재학부
박영환 ( Park Young-Hwan ) - 서울대학교 바이오시스템·소재학부
KMID : 0360120100320040293
Abstract
Purpose: The objective of the present study was to determine the capability of electrospun silk fibroin as a biomaterial template for bone formation when mixed with nano-hydoxyapatite in vivo.
Materials and Methods: Ten New Zealand white rabbits were used for this study and bilateral round shaped defects were formed in the parietal bone (diameter: 8.0 mm). The electrospun silk fibroin was coated by nano-hydroxyapatite and grafted into the right parietal bone (experimental group). The left side (control group) did not receive a graft. The animals were sacrificed at 6 weeks and 12 weeks, humanly. The microcomputerized tomogram (μCT) was taken for each specimen. Subsequently, they were undergone decalcification and stained for the histological analysis.
Results: The average value of all measured variables was higher in the experimental group than in the control at 6 weeks after the operation. BMC in the experimental group at 6 weeks after operation was 48.94 ± 19.25 and that in the control was 26.17 ± 16.40 (P = 0.027). BMD in the experimental group at 6 weeks after operation was 324.59 ± 165.24 and that in the control was 173.03 ± 120.30 (P = 0.044). TMC in the experimental group at 6 weeks after operation was 19.50 ± 6.00 and that in the control was 10.52 ± 6.20 (P = 0.011). TMD in the experimental group at 6 weeks after operation was 508.88 ± 297.57 and that in the control was 273.54 ± 175.91 (P = 0.06). Gross image of both groups showed higher calcification area at 12 weeks than them in 6 weeks. The average value of μCT analysis was higher at 12 weeks than that in 6 weeks in both groups. BMC in the experimental group at 12 weeks after operation was 51.21 ± 8.81 and that in the control was 33.47 ± 11.13 (P = 0.010). BMD in the experimental group at 12 weeks after operation was 323.39 ± 21.54 and that in the control was 197.75 ± 76.23 (P = 0.012). TMC in the experimental group at 12 weeks after operation was 21.44 ± 5.30 and that in the control was 13.31 ± 4.17 (P = 0.008). TMD in the experimental group at 12 weeks after operation was 524.47 ± 19.37 and that in the control was 299.60 ± 136.20 (P = 0.016).
Conclusion: The rabbit calvarial defect could be successfully repaired by electrospun silk nano-fiber combined with nano-hydroxyapatite.
키워드
Silk fibroin; Hydroxyapatite; Calvaria
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